Tuberculosis Test                                             
 
Test  Method
Step 1 :   
Add Reagent
 Step 2 : 
Add  Sample
 Step 3 :  
Second Reagent  Add 
 Step 4 : 
Read Result
  • Open the test card and lay

    •     it  flat on the work surface 
     
  • Add two drops of Reagent A 

    •    to the red/white conjugate 
       pad
  • Add 30 ul of sample on to

    •     the blue pad 
     
  • Wait until the serum or

    •    plasma has migrated to 
       the limit line
  • Add one drop of Reagent A 

    •    to the white pad
  • Close test card, wait and 

    •     read result through window
     
    Interpretation of Result 
    Positive 
    Any one or more PINK lines appears in the "T" area (irrespective of  its INTENSITY) is POSITIVE. 

    Negative 
    No PINK line in the "T" area is Negative 

    Invalid Test Result 
    If there is NO PINK line in the "C" area, you should repeat test again. If problem persist, contact  your local supplier.

    		 Test Principle   

    The ICT Tuberculosis test is an in vitro immunodiagnostic tool for the detection of antibodies to Mycobacterium tuberculosis in serum, plasma and other body fluids.  

    The test utilises 5 highly purified antigens which are secreted by Mycobacterium tuberculosis during an active infection. These antigens are immobilised on 4 lines across the test strip. When 30ul of serum or plasma is added to the blue pad, it diffuses past and binds to these antigen lines if human IgG against TB are present. Upon closure of the test card, anti-human IgG labelled with ‘colloidal gold’ particles will diffuse binds to the bound human IgG antibodies producing one or more PINK lines. If no specific antibodies are present in the sample, NO pink lines (except for the control) will be visible in the test area. 
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